Table 2.
The dsbA and dsbD mutations increased sensitivity to oxidative stress
| Strain | Genotype | Zone of growth inhibition (mm) ± SDa with: | ||
|---|---|---|---|---|
| H2O2 (1%) | Paraquat (100 mM) | Copper (100 mM) | ||
| NMB | wild type | 35.2 ± 1.1 | 30.0 ± 1.4 | 20.4 ± 0.9 |
| PKT107 | dsbA1−dsbA2− | 36.6 ± 1.3 | 34.0 ± 2.5* | 31.2 ± 1.1** |
| PKT108 | dsbA1−dsbA2−dsbD− | 42.8 ± 1.1**(**) | 39.2 ± 3.0**(*) | 36.0 ± 0.0**(**) |
| PKT113 | dsbA1−dsbA2−dsbD−; Plac::dsbD | 37.2 ± 1.79 | 34.0 ± 2.5* | 30.4 ± 0.9** |
a
Data are the means ± standard deviations growth inhibition zones around at least four 6-mm filter discs obtained with the indicated concentration of reagents after overnight incubation. A Student’s t test showed significant differences when compared to the wild type strain (*, p < 0.05; **, p < 0.01), while the statistically significant differences when compared to the dsbA1A2 strain are shown in parentheses.