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. Author manuscript; available in PMC: 2011 Nov 5.
Published in final edited form as: Nature. 2011 Apr 6;473(7345):101–104. doi: 10.1038/nature09881

Figure 3. Nkx2-1 controls lung adenocarcinoma differentiation and restricts metastatic ability.

Figure 3

a, Nkx2-1 protein expression in TMet cells. b, Nkx2-1 expression reduces lung nodule formation after intravenous transplantation. p < 0.002. c, Quantification of Nkx2-1 in lung nodules after TMet or TMet-Nkx2-1 transplantation. n=3/group. d, Association of Nkx2-1 expression with differentiation state after TMet or TMet-Nkx2-1 transplantation. Fisher’s exact test on the association of differentiation state with Nkx2-1 p < 0.002. e, Nkx2-1 expression reduces anchorage-independent growth of TMet cells. Representative images and colony number (mean +/− SD of quadruplicate wells, p < 0.0001). f, Nkx2-1 knockdown increases liver nodules after intrasplenic injection (top) and lung nodules after intravenous transplantation (middle) of TnonMet cells. Representative of 7 mice/group. shNkx2-1 enhanced anchorage-independent growth of TnonMet cells (bottom). Representative images and colony number (mean +/− SD of triplicate wells, p < 0.0001). g. Induction of tumours in KrasLSL-G12D/+;p53flox/flox mice with Nkx2-1/Cre lentivirus reduces the development of advanced tumours (grades 3&4). Numbers indicate percent of tumours in each group.