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. Author manuscript; available in PMC: 2011 Nov 5.
Published in final edited form as: Nature. 2011 Apr 6;473(7345):101–104. doi: 10.1038/nature09881

Figure 4. Nkx2-1 regulates the expression of Hmga2 in advanced lung adenocarcinoma.

Figure 4

a, Nkx2-1 knockdown derepresses Hmga2 in TnonMet cell lines. b, Hmga2 and Nkx2-1 are reciprocally expressed in KrasG12D/+;p53Δ/Δ murine lung adenocarcinomas. Scale bar = 50μm. Inlaid images show cellular features and protein localization. c, Hmga2 and Nkx2-1 expression in advanced KrasG12D/+;p53Δ/Δ murine lung adenocarcinomas. Fisher’s exact test, p-value < 10−11. d, Early KrasG12D/+;p53Δ/Δ tumours are Nkx2-1posHmga2neg. e, NKX2-1 and HMGA2 expression in human lung adenocarcinomas. Large numbers are percentages. Small numbers are absolute numbers. f, Hmga2 knockdown reduces the tumourigenic potential of TnonMet-shNkx2-1 cells after intravenous transplantation. Control samples include the parental TnonMet-shNkx2-1 cells (grey circle) and cells infected by a control retrovirus (black circles). p < 0.003. g, shHmga2 reduces anchorage-independent growth of a metastasis-derived cell line (Met). Representative images and colony number (mean +/− SD of quadruplicate wells, p < 0.0001). h, shHmga2 reduces the tumour-seeding potential of a Met cell line after intravenous transplantation. p <0.0001.