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. Author manuscript; available in PMC: 2011 Nov 5.
Published in final edited form as: Nature. 2011 Apr 10;473(7345):105–108. doi: 10.1038/nature09908

Figure 2.

Figure 2

Gld2 knockdown increases CPEB reporter mRNA and translation by a post-transcriptional mechanism. (a) Fold change of nuclear (intron-containing) or predominantly cytoplasmic (exon-containing) CPEB RNA following Gld2 depletion (n=3, bars are s.e.m.) (b) Schematic of reporter constructs used in the following experiments (numbers refer to nucleotides of CPEB 3’UTR). (c and d) Cells expressing firefly luciferase (Fluc) as a control and Renilla luciferase (Rluc) as noted in panel b were depleted of Gld2; the amount of Renilla lucifease activity (relative to firefly) was derived from RNA containing the entire CPEB 3’UTR (full) and set at 100. In all panels, n = 3 and the bars refer to s.e.m.; one or two asterisks refer to statistical significance (Students t test) at the p<0.05 or p<0.01 levels, respectively.