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. Author manuscript; available in PMC: 2011 Nov 5.
Published in final edited form as: Nature. 2011 Apr 10;473(7345):105–108. doi: 10.1038/nature09908

Figure 4.

Figure 4

Gld4 controls p53 mRNA expression. (a) p53 and actin western blots from fibroblasts expressing GFP (mock), shGld4, or shMitoPAP (shMPAP). (b) Fibroblasts containing shTETR or CPEB were transfected with Gld4-FLAG followed by FLAG antibody or IgG immunoprecipitation of RNA complexes and RT-PCR for p53 or GAPDH (control) RNAs. (c) Protein from fibroblasts infected with Gld4-HA and CPEB-FLAG was FLAG or IgG immunoprecipitated and western blotted for HA. Other cells infected with CPEB-FLAG and MCl1-HA (a nonspecific control) were processed similarly. (d) Examination of p53 poly(A) tail6 from skin fibroblasts expressing GFP or (mock) Gld4 or mitoPAP shRNAs. (e) RT-PCR analysis of predominantly cytoplasmic p53 RNA (exon-specific primers), or nuclear p53 pre-mRNA (intron-specific primers) in cells expressing GFP (mock) or Gld4 or MitoPAP shRNAs. (f) Model for regulation of p53 translation; see text for explanation.