Fig. 1.

Vitamin D3 is a potent inhibitor of cellular proliferation and Gli1 mRNA (n = 3 experiments). (A) Cellular proliferation studies were conducted in a BCC cell line (ASZ), non-tumorigenic keratinocytes (C5N), and a medulloblastoma cell line (Med1) incubated for 48 h with cyclopamine (CPN) versus 1,25(OH)₂D and vitamin D3. Mean±SEM, *P < 0.01 compared to C5N. (B) Cellular proliferation was assayed in BCC cell lines (ASZ, BSZ, CSZ) and non-tumorigenic keratinocytes (C5N) 48 h after treatment. Mean±SEM, *P < 0.01 compared to C5N. (C) 24-hydroxylase mRNA relative expression in ASZ cells treated with 7DHC (10 µM), 25(OH)D (10 µM), 1,25(OH)₂D (0.1 µM), vitamin D3 (10 µM), and CPN (10 µM) at 24 and 48 h. Mean±SEM, *P < 0.01 compared to control (DMSO or EtOH). (D) Gli1 mRNA relative expression in ASZ cells treated with 7DHC (10 µM), 25(OH)D (10 µM), 1,25(OH)₂D (0.1 µM), vitamin D3 (10 µM), and CPN (10 µM) at 24 and 48 h. Mean±SEM, *P < 0.01 compared to control (DMSO or EtOH).