Fig. 3.

Fig. 3.1. Histology of BCCs treated topically with acetone (control) for 30 days (A–F) or with topical 1.3 mg/kg vitamin D3 for 30 days (G–L). β-gal and hematoxylin and eosin staining for acetone or vitamin D3 treated BCC (Panel A, G). BCCs from Ptch1+/− K14-Cre-ER p53fl/fl mice stain blue due to β-galactosidase activity which is encoded by the lacZ gene that was inserted to replace the wildtype Ptch1 gene. Keratinocyte markers of differentiation, K14 (Panel B, H) and K10 (Panel C, I) are shown as well as CC3, a marker of apoptosis (Panel D, J) and Ki67 for proliferation (Panel E, F, K, L). Scale bars, 100 µm. Fig. 3.2. Levels of Ki67 staining in BCC tumors treated with either acetone (control, n = 7) or vitamin D3 (1.3 mg/kg, n = 10) for 30 days (P < 0.05).