Table 1.

Primers for EBV multiplex PCR

Target gene	Sequence	Length	Location in genomic gene	References
BNRF1	Sense 5′-GGAACCTGGTCATCCTTGC-3′	74 bp	4733–4753	(Niesters et al. 2000)
	Anti-sense 5′-ACGTGCATGGACCGGTTAAT-3′		4695–4679
EBNA2	Sense 5′-CCAGAGGTAAGTGGACTT-3′	124 bp	14614–1463	(Saito et al. 1989)
	Anti-sense 5′-GACCGGTGCCTTCTTAGG-3′		14735–14718
EBNA1	Sense 5′-GTCATCATCATCCGGGTCTC-3′	220 bp	96822–96841	(Telenti et al. 1990)
	Anti-sense 5′-TTCGGGTTGGAACCTCCTTG-3′		97091–97072
LMP1	Sense 5′-GACATGGTAATGCCTAGAAG-3′	259 bp	168130–168149	(Midgley et al. 2003)
	Anti-sense 5′-GCGACTCTGCTGGAAATGAT-3′		168389–16
BMLF	Sense 5′-CACCACCTTGTTTTGACGGG-3′	304 bp	83037–83056	(Pedneault and Katz 1993)
	Anti-sense 5′-GTCAACCAACAAGGACACAT-3′		83340−83321
EBNA3C	Sense 5′-CAATCGCACCTGCAAGCGCTA-3′	518 bp	100362–100383	(Midgley et al. 2003)
	Anti-sense 5′-GACACCCATGAAACGCACGAAATC-3′		100806–100785
BZLF1	Sense 5′-ATTGCACCTTGCCGCCACCTTTG-3′	608 bp	103194–103180	(Luo et al. 2005)
	Anti-sense 5′-CGGCATTTTCTGGAAGCCACCCGA-3′		102486–102463
ALAS1	Sense 5′-CTGGGGCAGGTGGTACTAGA-3′	804 bp	1161–1180	Designed by Primer3 input0.4.0
	Anti-sense 5′-TCATTGTGGCGGAAGATGTA-3′		1417–1397	online software