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Measurement of female B. pahangi worm fecundity in vitro. At necropsy, two female B. pahangi adult worms were isolated from each gerbil. Worms were incubated overnight in 2 ml of RPMI medium. Enumeration of microfilariae (A), eggs (B), and DE (C) was carried out once each with two 20-μl aliquots of the culture. Data are presented as the means of these two counts.
