FIG. 5.

The effect of the E:T ratio on type 8-stimulated IL-8 and secretion from human PMNs. The amount of IL-8 (A) and IL-6 (B) in supernatants secreted by PMNs cocultured with killed type 8 pneumococci (P), D11, NAD, or the isotype controls (IgM or IgA) with HS was determined by ELISA. Symbols in panel A: *, P < 0.001, comparing D11 to IgM; **, P < 0.05, comparing NAD to IgA. Symbol in panel B: *, P < 0.05 comparing D11 to IgM. The error bars represent standard deviations of replicate samples. All statistical comparisons were performed with a one-way ANOVA and the Bonferroni multiple comparison test.