TABLE 3.
IL-6 levels in 18-h cocultures of human PMNs, type 8 pneumococci, human complement sources, and human IgM and IgA MAbs to type 8 PS
| Conditiona | IL-6 (ng/ml)b
|
||||
|---|---|---|---|---|---|
| Expt 1
|
Expt 2
|
Expt 3
|
|||
| Unheated | Heated | Unheated | Unheated | Heated | |
| P/PMN/FB−/no MAb | 2.2 ± 0.4 | 1.3 ± 0.5 | 2.5 ± 2.1 | 2.6 ± 1.1 | 3.0 ± 1.5 |
| P/PMN/FB−/D11 | 5.0 ± 0.8 | 5.0 ± 0.9 | 6.1 ± 1.0 | 3.1 ± 1.1 | 2.8 ± 1.0 |
| P/PMN/FB−/IgM | 5.0 ± 0.6 | 4.5 ± 3.7 | 3.4 ± 1.1 | 2.4 ± 1.2 | 3.9 ± 1.4 |
| P/PMN/C2−no MAb | 1.8 ± 0.4 | 0.7 ± 0.5 | 1.6 ± 1.7 | 1.7 ± 0.7 | 1.3 ± 1.2 |
| P/PMN/C2−/D11 | 5.2 ± 0.8 | 3.7 ± 2.3 | 7.3 ± 1.8 | 1.9 ± 1.8 | 3.3 ± 1.3 |
| P/PMN/C2−/IgM | 4.0 ± 0.6 | 3.2 ± 2.8 | 7.9 ± 2.4 | 2.6 ± 1.4 | 3.1 ± 1.2 |
| P/PMN/HS/no MAb | 2.8 ± 0.4 | 2.2 ± 2.4 | 3.0 ± 1.4 | 2.0 ± 1.6 | 3.2 ± 2.5 |
| P/PMN/HS/D11 | 4.0 ± 1.8d | 7.5 ± 0.5c | 4.8 ± 1.8 | 3.2 ± 1.4 | 3.6 ± 1.6 |
| P/PMN/HS/IgM | 5.7 ± 2.3 | 3.8 ± 0.4 | 6.0 ± 1.2 | 3.2 ± 1.4 | 2.9 ± 1.4 |
| P/PMN/FB−/no MAb | 1.0 ± 0.9 | 1.1 ± 0.5 | 2.5 ± 2.1 | 2.6 ± 1.1 | 3.0 ± 1.5 |
| P/PMN/FB−/NAD | 4.0 ± 1.0 | 3.0 ± 0.5 | 3.3 ± 1.6 | 3.2 ± 1.6 | 3.5 ± 1.4 |
| P/PMN/FB−/IgA | 3.7 ± 0.6d | 2.2 ± 0.4 | 4.7 ± 2.3 | 3.1 ± 1.3 | 3.1 ± 1.5 |
| P/PMN/C2−/no MAb | 2.6 ± 0.5 | 0.3 ± 0.3 | 1.6 ± 1.7 | 1.7 ± 0.7 | 1.3 ± 1.2 |
| P/PMN/C2−/NAD | 5.7 ± 1.0d | 2.7 ± 0.5 | 5.8 ± 0.8 | 2.7 ± 1.7 | 3.4 ± 1.5 |
| P/PMN/C2−/IgA | 5.7 ± 0.9d | 2.0 ± 0.5 | 6.0 ± 1.7 | 2.2 ± 1.0 | 3.6 ± 1.9 |
| P/PMN/HS/no MAb | 3.5 ± 0.7 | 1.4 ± 0.9 | 3.0 ± 1.4 | 2.0 ± 1.6 | 3.2 ± 2.5 |
| P/PMN/HS/NAD | 5.0 ± 0.6c | 5.0 ± 1.0c | 5.5 ± 1.3 | 3.3 ± 1.3 | 2.8 ± 1.0 |
| P/PMN/HS/IgA | 6.2 ± 0.8d | 3.3 ± 0.5 | 7.1 ± 2.7 | 3.1 ± 1.4 | 2.0 ± 1.6 |
a
P, type 8 pneumococci; PMN, human PMNs; FB−, factor B-deficient serum; C2−, C2-deficient serum; HS, human serum.
b
IL-6 levels in secreted supernatants from cocultures performed with unheated and heat-inactivated complement sources; values represent mean ± standard deviation of duplicate samples for each condition.
c
P < 0.05 comparing type-specific MAb to isotype control MAbs, using one-way ANOVA and Bonferroni multiple comparison test.
d
P < 0.05 comparing conditions with heated and unheated complement sources, using one-way ANOVA and Bonferroni multiple comparison test.