Table 3.
Expression rates of proliferation marker Ki-67 in DCIS
| Biomarker | First author and reference | Year | No. of samples | Expression rate, % | Comments |
|---|---|---|---|---|---|
| Ki-67 | |||||
| Albonico 7 | 1998 | 62 | 3.4-65.4 | Tumors with nuclear staining in more than 13% of cells were scored as positive. 65.4 (comedo DCIS) 7.5 (noncomedo DCIS) 3.4 (cribriform DCIS) | |
| Ringberg 16 | 2001 | 187 | 42 | 42% of cases exhibited staining in more than 10% of the tumor cells. | |
| Menter 72 | 2001 | 200 | 0.99-2.59 | Ki-67 labeling index was determined by computerized image analysis. The range of Ki-67 labeling index in DCIS nuclear grades I-III was 0.99-2.59. | |
| Wärnberg 36 | 2001 | 194 | 19 | 19% of cases exhibited staining in at least 10% of the tumor cells. | |
| Lebeau 12 | 2003 | 45 | 45.5 | 45.5% of cases exhibited staining in more than 10% of the tumor cells. | |
| Boland 19 | 2004 | 187 | 49.2 | The percentage of positively stained nuclei (at least 1,000 cells were counted for each case) was determined using a grid graticule and cell counter at X400 magnification. 50.8% of cases exhibited staining in at least 10% of tumor cells. | |
| Barnes 35 | 2005 | 129 | 10.9 (nonrecurrent DCIS);15.5 (recurrent DCIS) | The percentage of positively stained nuclei (at least 1,000 cells were counted for each case) was determined using a grid graticule and cell counter at X400 magnification. The median percentage of cells with Ki-67 expression was 10.9-15.5% | |
| Barnes 54 | 2006 | 161 | 8.7-14.4 | The percentage of positively stained nuclei (at least 1,000 cells were counted for each case) was determined using a grid graticule and cell counter at X400 magnification. The median percentage of cells with Ki-67 expression was 8.7-14.4%. | |
| Wilson 37 | 2006 | 129 | 50.4 | The percentage of positively stained nuclei (at least 1.000 cells were counted for each case) was determined using a grid graticule and cell counter at X400 magnification. 50.4% of cases exhibited staining in at least 10% of tumor cells. | |
| Gauthier 45 | 2007 | 70 | 37.1 | 37.1% of cases exhibited staining in more than 10% of the tumor cells. | |
| Livasy 66 | 2007 | 245 | 36 | 36% of cases exhibited staining in more than 10% of the tumor cells. | |
| Okumura 52 | 2008 | 52 | 17.9 | The percentage of cancer cells with positively stained nuclei was determined. The mean percentage Ki-67 staining was 17.9 ± 1.5%. | |
| Altintas 15 | 2009 | 159 | 36 | 36% of cases exhibited staining in more than 10% of the tumor cells. | |
| Kuerer 18 | 2010 | 69 | 44.29 ± 3.42 | No cut-off value was mentioned in the paper. The mean (± standard deviation) percentage of cells with Ki-67 staining was 44.29 ± 3.42%. Only patients with HER2-positive DCIS were included in the study. | |
| Kerlikowske 24 | 2010 | 329 | 47.5 | 47.5% of cases exhibited staining in more than 10% of the tumor cells, and thus 47.5% was set as the median value (positive cells divided by the number of positive plus negative cells) to divide cases with low and high proliferative activity. | |
| Bundred 30 | 2010 | 90 | 13.5-20.7 | Ki-67 scores were calculated as the percentage of positively stained nuclei. The median percentages in each of the different treatment subgroups in this randomized trial were reported before patients were given aromatase inhibitor therapy. All patients had ER-positive DCIS. | |