Table 4.
Expression rates of cell cycle regulation and apoptotic markers in DCIS
| Biomarker | First author and reference | Year | No. of samples | Expression rate, % | Definition of positivity |
|---|---|---|---|---|---|
| cyclin D1 | |||||
| Oh 41 | 2001 | 49 | 59.2 | Moderate or strong nuclear staining in more than 10% of tumor cells. | |
| Lebeau 12 | 2003 | 45 | 48.8 | Nuclear staining in more than 10% of tumor cells. | |
| Chasle 38 | 2003 | 50 | 37 | The percentage of marked nuclei was determined for 300-400 nuclei in the most positive foci. The mean for cyclin D1 was reported to be 37%. | |
| Millar 31 | 2007 | 60 | 58 | Nuclear staining in more than 5% of tumor cells. | |
| Okumura 52 | 2008 | 52 | 70.6 | Nuclear staining in more than 10% of tumor cells. | |
| Kulkarni 21 | 2008 | 69 | 61.5 | Nuclear staining in at least 10% of tumor cells. | |
| cyclin A | |||||
| Millar 31 | 2007 | 60 | 35 | Nuclear staining in more than 10% of tumor cells. | |
| cyclin E | |||||
| Jirström 42 | 2003 | 92 | 25 | A mean value was used as a cut-off to divide expression of cyclin E. | |
| p16 | |||||
| Jirström 42 | 2003 | 92 | 37.5 | A mean value was used as a cut-off to divide expression of p16. | |
| Gauthier 45 | 2007 | 70 | 28 | Allred score of at least 2. | |
| Kerlikowske 24 | 2010 | 329 | 39.3 | On a 3-point scale, a score of at least 2. | |
| p21 | |||||
| Oh 41 | 2001 | 49 | 67.3 | Moderate or strong nuclear staining in more than 10% of tumor cells. | |
| Provenzano 22 | 2003 | 95 | 34 | On a 6-point scale, a score of 4-6 (moderate or strong staining). | |
| Lebeau 12 | 2003 | 45 | 42.2 | Nuclear staining in more than 10% of tumor cells. | |
| Chasle 38 | 2003 | 50 | 76 | The percentage of marked nuclei was determined for 300-400 nuclei in the most positive foci. | |
| Okumura 52 | 2008 | 52 | 69.2 | Nuclear staining in more than 10% of tumor cells. | |
| Kulkarni 21 | 2008 | 69 | 29.6 | Nuclear staining in at least 10% of tumor cells. | |
| p27 | |||||
| Oh 41 | 2001 | 49 | 46.9 | Moderate or strong nuclear staining in more than 10% of tumor cells. | |
| Jirström 42 | 2003 | 92 | 68.5 | Nuclear and cytoplasmic staining intensity were evaluated using a 3-point semiquantitative scoring scale (0=none, 1=weak, 2=moderate, 3=strong). | |
| Millar 31 | 2007 | 60 | 55 | Nuclear staining in more than 50% of tumor cells. | |
| p53 | |||||
| Albonico 7 | 1998 | 62 | 7.0-37.3 | Nuclear staining in more than 5% of tumor cells. 37.3 (comedo DCIS) 17.2 (noncomedo DCIS) 7.0 (cribriform DCIS) | |
| Bijker 10 | 2001 | 116 | 20 | On an 8-point scale, a score of at least 5. | |
| Ringberg 16 | 2001 | 187 | 26 | Nuclear staining in more than 10% of tumor cells. | |
| Hieken 48 | 2001 | 103 | 27 | Strong nuclear staining in more than 10% of tumor cells. | |
| Wärnberg 36 | 2001 | 194 | 40 | Any p53 expression. | |
| Oh 41 | 2001 | 49 | 30.6 | Nuclear staining in more than 5% of tumor cells. | |
| Provenzano 22 | 2003 | 95 | 60 | On a 6-point scale, a score of 4-6 (moderate or strong staining). | |
| Lebeau 12 | 2003 | 45 | 25 | Moderate or strong nuclear staining in more than 40% of tumor cells. | |
| Chasle 38 | 2003 | 50 | 88 | The percentage of marked nuclei was determined for 300-400 nuclei in the most positive foci. | |
| Roka 23 | 2004 | 190 | 57.2 | Nuclear staining in at least 10% of tumor cells. | |
| Perrone 20 | 2005 | 49 | 26.5 | Nuclear staining in more than 10% of tumor cells. | |
| Kepple 59 | 2006 | 94 | 50 | No cut-off value was mentioned in the paper. | |
| Livasy 66 | 2007 | 245 | 31 | Nuclear or nuclear and cytoplasmic staining in more than 10% of tumor cells. | |
| Meijnen 14 | 2008 | 163 | 26 | Nuclear staining in more than 25% of tumor cells. | |
| Okumura 52 | 2008 | 52 | 76.5 | Nuclear staining in more than 10% of tumor cells. | |
| Kulkarni 21 | 2008 | 69 | 67.9 | Nuclear staining in at least 10% of tumor cells. | |
| Kerlikowske 24 | 2010 | 329 | 11.3 | Nuclear staining in at least 10% of tumor cells. | |
| Bcl-2 | |||||
| Albonico 7 | 1998 | 62 | 35.7-100 | Nuclear staining in more than 10% of tumor cells. 35.7 (comedo DCIS) 100 (noncomedo DCIS) 100 (cribriform DCIS) | |
| Ringberg 16 | 2001 | 187 | 56 | Cytoplasm staining in more than 10% of tumor cells. | |
| Wärnberg 36 | 2001 | 194 | 48 | Nuclear staining in at least 10% of tumor cells. | |
| Provenzano 22 | 2003 | 95 | 50 | On a 6-point scale, a score of 4-6 (moderate or strong staining). | |
| Meijnen 14 | 2008 | 163 | 64 | Weak cytoplasmic staining in more than 10% of tumor cells. | |
| Okumura 52 | 2008 | 52 | 58.8 | Nuclear staining in more than 5% of tumor cells. | |
| Bax | |||||
| Okumura 52 | 2008 | 52 | 71.2 | Nuclear staining in more than 5% of tumor cells. | |
| Survivin | |||||
| Barnes 54 | 2006 | 161 | 10 (nuclear staining alone);29% (cytoplasmic staining alone);29% (cytoplasmic and nuclear staining) | Survivin staining was scored for both cytoplasmic and nuclear staining. For cytoplasmic staining, a score of at least 2 was considered positive; for nuclear staining, the proportion of positive cells out of at least 1,000 was determined. | |
| Okumura 52 | 2008 | 52 | 55.8 | Nuclear staining in more than 5% of tumor cells. | |
| C-myc | |||||
| Altintas 15 | 2009 | 159 | 60 | Score of at least 2. | |
| Rb | |||||
| Okumura 52 | 2008 | 52 | 68.6 | Nuclear staining in more than 10% of tumor cells. | |