Figure 4.

The biologically more-aggressive OSA cell lines demonstrate increased double-strand DNA damage. In all cell types nuclear phosphorylated H2AX foci are stained red with DAPI counterstain. γH2AX immunocytochemistry demonstrates that Abrams, D17, Grey, Hughes, and U2 exhibit more nuclear foci of phosphorylated γH2AX as compared to intermediate staining within Marisco and the less aggressive canine OSA cell lines (Ingles and Jarques). U2 cells have the highest level of nuclear HSAX staining compared to all other cell lines. Representative images from the following osteosarcoma cell lines are within the panels indicated: A) Abrams, B) D17, C) Grey, D) Hughes, E) Ingles, F) Jarques, G) Marisco, H) U2.