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. 2011 May 4;2:262–270. doi: 10.7150/jca.2.262

Figure 5.

Figure 5

The biologically more-aggressive OSA cell lines demonstrate increased mitochondrial and nuclear oxidative evidence characterized by increased DNA 8-oxoguanine. In all cell types mitochondrial and nuclear 8-oxoguanine foci are stained green with DAPI for nuclear counterstain. Abrams, D17, Grey, and Marisco demonstrate increased oxidative damage as evidenced by increased mitochondrial and nuclear staining of 8-oxoguanine. Hughes demonstrates intermediate staining, while the less aggressive canine OSA cell lines (Ingles and Jarques) and the human OSA cell line (U2) demonstrate minimal staining of 8-oxoguanine. Representative images from the following osteosarcoma cell lines are within the panels indicated: A) Abrams, B) D17, C) Grey, D) Hughes, E) Ingles, F) Jarque, G) Marisco, H) U2.