FIG. 1.

Activation of complement in vitro by H. felis. Serum from control WT or Rag2^−/− mice was incubated with H. felis suspension or LPS for various lengths of time, and C3 levels were subsequently measured by ELISA at various time points. The results are expressed as OD₄₉₂ ± SD and represent the mean values of the results for a triplicate of a given sample. LPS (positive control), rapidly activated complement; NMS, normal mouse serum; RMS, Rag2^−/− mouse serum. An asterisk (*) indicates a P value of <0.001 compared to the results for the control, and a number sign (^#) indicates a P value of <0.001 compared to the results for normal mouse serum incubated with H. felis. The results are representative of three independent experiments.