TABLE 3.
Primers used in RT-PCR analysis
| Primer | Primer sequence (5′→3′) | RT-PCR product size (bp) |
|---|---|---|
| β-Actin I | TGG AAT CCT GTG GCA TCC ATG AAA C | 348 |
| β-Actin II | TAA AAC GCA GCT CAG TAA CAG TCC G | |
| IL-1β I | TCA TGG GAT GAT GAT GAT AAC CTG CT | 502 |
| IL-1β II | CCC ATA CTT TAG GAA GAC ACG GAT T | |
| IL-6 I | CTG GTG ACA ACC ACG GCC TTC CCT A | 600 |
| IL-6 II | ATG CTT AGG CAT AAC GCA CTA GGT T | |
| IL-12 I | CGT GCT CAT GGC TGG TGC AAA G | 312 |
| IL-12 II | CTT CAT CTG CAA GTT CTT GGG C | |
| IFN-γ I | AGC GGC TGA CTG AAC TCA GAT TGT AG | 243 |
| IFN-γ II | GGC AGG TCT ACT TTG GAG TCA TTG C | |
| TNF-α I | GGC AGG TCT ACT TTG GAG TCA TTG C | 307 |
| TNF-α II | ACA TTC GAG GCT CCA GTG AAT TCG G | |
| iNOS I | ACG CTT GGG TCT TGT TCA CT | 468 |
| iNOS II | GTC TCT GGG TCC TCT GGT CA |