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. 2003 Dec;71(12):7002–7013. doi: 10.1128/IAI.71.12.7002-7013.2003

TABLE 3.

Primers used in RT-PCR analysis

Primer Primer sequence (5′→3′) RT-PCR product size (bp)
β-Actin I TGG AAT CCT GTG GCA TCC ATG AAA C 348
β-Actin II TAA AAC GCA GCT CAG TAA CAG TCC G
IL-1β I TCA TGG GAT GAT GAT GAT AAC CTG CT 502
IL-1β II CCC ATA CTT TAG GAA GAC ACG GAT T
IL-6 I CTG GTG ACA ACC ACG GCC TTC CCT A 600
IL-6 II ATG CTT AGG CAT AAC GCA CTA GGT T
IL-12 I CGT GCT CAT GGC TGG TGC AAA G 312
IL-12 II CTT CAT CTG CAA GTT CTT GGG C
IFN-γ I AGC GGC TGA CTG AAC TCA GAT TGT AG 243
IFN-γ II GGC AGG TCT ACT TTG GAG TCA TTG C
TNF-α I GGC AGG TCT ACT TTG GAG TCA TTG C 307
TNF-α II ACA TTC GAG GCT CCA GTG AAT TCG G
iNOS I ACG CTT GGG TCT TGT TCA CT 468
iNOS II GTC TCT GGG TCC TCT GGT CA