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Western blotting analysis of the expression of recombinant plasmids and β-actin. (A)Samples were harvested from cell lysates at 48 h after transfection. prME-WT, prME-M1, PVAX-prME-M2, and PVAX-prME-M3 proteins were incubated with PNGase F (+) or buffer only (−). (B) β-actin was used as internal control.
