TABLE 2.

Quantitation of chemokine, cytokine, and mycobacterial RNA expression in granulomatous lesions of cynomolgus macaques experimentally infected with M. tuberculosis

Lesions	Disease severity	Animal	No. of granulomas	Granuloma size (μm2)a	No. of myco- bacterial rRNA collectionsb	No. of IFN-γ mRNA+ cellsc	No. of TNF-α mRNA+ cellsc	CXCL9/ Mig ISH signal (%)d	CXCL10/ IP-10 ISH signal (%)d	CXCL11/ I-TAC ISH signal (%)d
Solid	Minimal or moderate	M15000	12	184,882	0.9	38.2	31.2	23.54	35.89	8.47
		M15100	6	157,386	0.0	6.5	2.5	9.54	6.83	1.56
		Avg		175,717	0.8	27.0	21.1	18.29	17.58	6.17
	Advanced	M7200	4	189,597	2.0	46.0	NAe	26.96	19.37	31.07
		M11201	12	135,894	NA	2.8	3.1	55.68	43.10	6.82
		M11301	9	131,056	1.4	39.4	11.2	49.53	42.59	27.11
		M15300	56	337,018	3.9	20.6	14.1	57.15	28.37	15.87
		Avg		277,057	3.5	23.1	11.6	55.02	32.08	17.15
	Allf			258,631	2.8	24.1	14.0	48.03	29.15	14.48
						(0-156)	(0-130)	(1.33-84.22)	(0.16-90.53)	(0.08-75.44)
Caseous	Minimal or moderate	M7100	2	1,122,068	NA	114.0	42.0	40.23	23.48	10.01
		M15000	1	996,507	0.0	47.0	NA	30.60	22.60	0.26
		M15100	1	1,020,000	NA	20.0	10.0	16.86	8.17	1.99
		Avg		1,065,161	0.0	60.3	26.0	29.23	18.09	5.57
	Advanced	M7200	3	875,262	4.3	92.3	NA	32.61	27.14	22.95
		M11201	12	591,442	NA	39.6	13.0	60.11	41.27	12.88
		M11301	6	634,611	246.0	112.8	28.8	51.93	44.15	22.16
		M15300	177	678,735	4.1	49.1	28.0	51.32	32.75	11.52
		Avg		675,010	45.17	51.8	26.9	51.58	33.53	12.00
	Allf			682,892	45.0	51.9	26.9	51.19	33.25	11.83
						(1-238)	(2-135)	(14.14-99.38)	(0.35-74.44)	(0.26-40.16)

^aAverage surface area of granulomas per 14-μm section.

^bAverage number of mycobacterial 16S rRNA focal collections per 14-μm section of granuloma.

^cAverage number of mRNA⁺ cells per 14-μm section of granuloma.

^dAverage percentage of cellular surface area comprised of autoradiographic silver grains.

^eNA, not available.

^fAverage (range) for all lesions in each group.