Table 2.
Primers sequence for PCR
| Gene | Reverse transcription primer | PCR primer |
|---|---|---|
| miR-146b | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGAGCCTATG; | F: ACACTCCAGCTGGGTGAGAACTGAATTC; |
| R: TGGTGTCGTGGAGTCG; | ||
| miR-155 | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACCCCTATC; | F: ACACTCCAGCTGGGTTAATGCTAATCGTGAT; |
| R: TGGTGTCGTGGAGTCG; | ||
| miR-1224 | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTCCACCT; | F: ACACTCCAGCTGGGGTGAGGACTGGGG; |
| R: TGGTGTCGTGGAGTCG; | ||
| U6 | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATATGG; | F: CTCGCTTCGGCAGCACA; |
| R: AACGCTTCACGAATTTGCGT; | ||
| TNF-α | F: CGAAGTGGTGGTCTTGTTG; R: CGAGTCTGGGCAGGTCTA; | |
| TLR4 | F: GCTTTCACCTCTGCCTTCAC; R: AGGCGATACAATTCCACCTG; | |
| GAPDH | F: GGTGAAGGTCGGTGTGAACG; R: CTCGCTCCTGGAAGATGGTG; | |
| TNF-Α promoter | F1: CGGGGAGGAGATTCCTTGATG; R1: CTGGCTGGCTGTGCAGACG | |
| F2: ATTATTTATTTATTTGCTTATGAATG; R2: CAGTGATGTAGCGACAGCCTG |
RT primer is stem-loop primer used for reverse transcription of a specific miRNA or U6.
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TLR, Toll-like receptor; TNF, tumour necrosis factor.