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. 2011 May;133(1):41–50. doi: 10.1111/j.1365-2567.2011.03407.x

Figure 3.

Figure 3

Peptidoglycan (PGN) -stimulated intracellular interleukin-12p70 (IL-12p70) and tumour necrosis factor (TNF) is higher in cord blood than in adult monocytes. Cord blood (CBMC; n = 20) or peripheral blood (PBMC; n = 10) mononuclear cells were stimulated for 24 hr with 1 μg/ml PGN in the presence of GolgiStop and CD14+ cells were analysed by flow cytometry for intracellular expression of IL-12p70 and TNF. (a) Representative forward and side scatter characteristics of CBMC (upper panel) or PBMC (lower panel). (b) Representative cytokine responses of cord blood (upper panel) or adult monocytes (lower panel). In (c) and (d), respectively, IL-12p70 and TNF levels in cord blood versus adult monocytes are shown. (e) IL-12p70 levels in cord blood and adult CD14++ CD16− (□) and CD14+ CD16+ monocytes (Inline graphic). Data are shown as geometric mean fluorescence intensity (GeoMFI) or in (f) as % CD14+ CD16+ cells of total CD14+ cells in PGN-stimulated cultures. Boxes cover 50% of the values between the 25th and the 75th percentiles, and whiskers extend to the 10th and 90th percentiles. Raw data are shown excluding outliers and extremes. The Mann–Whitney U-test was used to evaluate differences between groups.