Skip to main content
. 2011 May;133(1):41–50. doi: 10.1111/j.1365-2567.2011.03407.x

Figure 5.

Figure 5

Effect of soluble factors in serum on cytokine release. Cord blood (CBMC; n = 4) or peripheral blood (PBMC; n = 4) mononuclear cells were cultured for 24 hr with 1 μg/ml peptidoglycan (PGN) with either 10% autologous serum in culture media, or 10% sera from four different adult donors (for CBMC n = 4 × 4 combinations) or four different cord bloods (for PBMC n = 4 × 4 combinations). Supernatants were subsequently analysed by cytometric bead array for (a) interleukin-6 (IL-6) and IL-8, (b) IL-10 and tumour necrosis factor (TNF) and (c) IL-1β. For the purposes of comparison, data are shown as modulation index with the horizontal line at 1 representing cytokine levels in cultures with 10% fetal calf serum as serum source. Boxes cover 50% of the values between the 25th and the 75th percentiles, and whiskers extend to the 10th and 90th percentiles. Raw data are shown excluding outliers and extremes. The Mann–Whitney U-test was used to evaluate differences between groups.