TABLE 1.
M. tuberculosis (H37Rv)-derived H-2Kd epitopes predicted by use of the SYFPEITHI algorithm
| H37Rv proteinsa | Putative H-2Kd-binding amino acid sequenceb | Epitope positions | Computed score for H-2Kd |
|---|---|---|---|
| Membrane proteins (no. of transmembrane domains) | |||
| Rv0051 (9) | IYRPGRDLV | 487-495 | 23 |
| Rv0111 (10) | PYRYIGQTL | 509-517 | 23 |
| Rv0194 (9) | LYTPIGELA | 894-902 | 23 |
| Rv0219 (4) | SYGSGPLHL | 11-19 | 25 |
| Secreted proteins | |||
| Rv0288 (TB10.4), Rv3019c (TB10.3) | GYAGTLQSL | 20-28, 20-28 | 27 |
| Rv3804c (Ag85A) | VYAGAMSGLc | 144-152 | 25 |
| Proteins with unknown localizations | |||
| Rv0065 | RYPHSPRLI | 76-84 | 24 |
| Rv0127 | WYAGRNREL | 22-30 | 24 |
| Rv0140 | SYYSVTTDA | 79-87 | 27 |
| Rv0161 | RYRGRASAL | 31-39 | 26 |
| Rv0164 | RYASRMPVL | 11-19 | 24 |
| Rv0393, Rv0397 | TYVTTPGSA | 350-358, 27-25 | 25 |
a
Predicted proteins of M. tuberculosis H37Rv (http://genolist.pasteur.fr/TubercuList/).
b
Underlined letters represent H-2Kd consensus binding motifs.
c
A previously described Ag85A-derived epitope restricted by H-2Kd (3).