TABLE 2.
Cell differential counts for guinea pig BAL cells, PC, and SPC
| Vaccination status | Cell subtypea | Mean % ± SEM in the following guinea pig cell subpopulationb:
|
||
|---|---|---|---|---|
| BAL cells | PC | SPC | ||
| Unvaccinated | LMC | 57.8 ± 3.3 | 61.9 ± 9.1 | 11.3 ± 2.1 |
| Lymphocytes | 2.60 ± 0.7 | 3.10 ± 0.9 | 54.3 ± 1.7 | |
| Eosinophils | 39.0 ± 4.4 | 33.4 ± 7.7 | 3.70 ± 0.5 | |
| Neutrophils | 0.60 ± 0.1 | 1.60 ± 0.7 | 17.9 ± 2.5 | |
| Kurloff cells | 0.00 ± 0.0 | 0.00 ± 0.0 | 12.8 ± 3.1 | |
| BCG vaccinated | LMC | 49.5 ± 3.1 | 60.3 ± 1.1 | 9.80 ± 1.6 |
| Lymphocytes | 3.60 ± 1.3 | 4.00 ± 1.5 | 52.7 ± 5.5 | |
| Eosinophils | 45.5 ± 2.0 | 34.6 ± 0.9 | 4.10 ± 0.6 | |
| Neutrophils | 1.40 ± 0.5 | 1.10 ± 0.4 | 18.6 ± 4.9 | |
| Kurloff cells | 0.00 ± 0.0 | 0.00 ± 0.0 | 14.8 ± 1.2 | |
a
Cellular differentials were determined by morphology.
b
Cytospins were performed on BAL cells, PC, and SPC as described in Materials and Methods. One slide was prepared for each cell population per guinea pig per treatment group (n = 4); 250 cells per slide were enumerated, and erythrocyte contamination for BAL cell and PC preparations was consistently <5% for this study (lysed cells were not counted).