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Effect of cytokines on ZnT8 and ZIP4 mRNA levels in intact murine islets. ZnT8 (A) and ZIP4 (B) mRNA levels were determined in intact murine islets after treatment for 24 h with 5 ng/ml IL1β or 10 ng/ml TNFα. Values are the mean level of ZnT8 and ZIP4 mRNA compared with the level in control cells, which was defined as 1·0 and are the mean ±S.E.M. (n = 4 for ZnT8, n = 3 for ZIP4). *P<0·05, **P<0·005 compared with control.
