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. Author manuscript; available in PMC: 2012 Apr 5.
Published in final edited form as: Biochemistry. 2011 Feb 28;50(13):2550–2558. doi: 10.1021/bi1020106

Figure 5. Denaturation of pyrene labeled apoE measured by FRET and kinetics of lipidation monitored by FRET and turbidity clearance.

Figure 5

A) FRET between pyrene at position 102 and tryptophans in pyrene labeled apoE4 (A102C) as function of urea concentrations (squares). The solid line represents fit to the denaturation data using a two step model according to (37). B) Kinetics of lipidation of pyrene labeled apoE4 (A102C) as monitored by FRET (○) or by turbidity clearance (□). The solid line was calculated from the rate constants for apoE4 as shown in Table 1. The concentration of apoE4 used was 110 nM. FRET signal was monitored at 340 nm with excitation at 290 nm and scattering was monitored at 600 nm.