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. 2011 Feb 11;2:19. doi: 10.3389/fmicb.2011.00019

Figure 3.

Figure 3

(A) Expression of Rv1639c in M. tuberculosis lysX strains. Expression of Rv1639c was evaluated by real-time PCR using RNA isolated from Wild-type M. tuberculosis (WT), the lysX mutant (Mut) and both the Pami (Ami) and Ptet (Tet) complement strains, and results were normalized against expression of 16s rRNA as described above. All values are the means ± SD from two independent experiments with triplicate samples; single star represents P < 0.01 and double stars indicate P < 0.001 versus WT (Student's t-test). (B) Antibiotic sensitivity of M. smegmatis merodiploid strains overproducing Rv1639 and LysX proteins. Cultures were grown in the presence or absence of either (A) Polymyxin B 130 and 195 U/mL, or (B) vancomycin 5 μg/mL, 10 μg/mL (VAN5, VAN10). Bars represent absorbance values for each culture. The values represented are the means ± SD for four independent samples; stars represent P < 0.01 compared with the control culture (-) for each (Student's t-test).