Figure 8.

Turnover of M. tuberculosis phospholipids following ¹⁴C-acetic acid incorporation. Total lipids were extracted and analyzed by TLC. Radioactive spots corresponding to each phospholipid were identified, lipids were extracted and radioactivity present in each spot was measured by scintillation counting. Closed symbols stand for ΔlysX and open ones correspond to RvWT. (A) represents changes in the level of PI, (B) PE, and (C) CL. Data are presented as percentage of total lipids, relatively to time zero. Values were obtained from two independent experiments in duplicate. The star represents P < 0.02 and hash P < 0.05 (t-test method). The values represent averages and bars indicate the SD.