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. Author manuscript; available in PMC: 2011 May 6.
Published in final edited form as: Mol Biochem Parasitol. 2005 Oct;143(2):135–145. doi: 10.1016/j.molbiopara.2005.05.009

Fig. 2.

Fig. 2

Gene copy number of LRNase HIIC. Southern blot analysis of the restriction digests of total DNA isolated from L. donovani (A) and L. major (B). Total DNA (5 μg for L. donovani and 10 μg for L. major) was digested with R1, Eco RI; H, Hind III; P, Pst I; Sc, Sac II; Sl, Sal I; Sm, Sma I and Sp, Spe I. The fragments were analyzed by agarose gel (0.8%) electrophoresis. The DNA fragments from the gel were transferred to Nytran membrane and hybridized with 32P-labeled RNase HII cDNA as probe. One kb plus DNA ladder (Invitrogen) was used as molecular size marker. Upper panels show the photographs of ethidium bromide-stained agarose gels and the lower panels show the corresponding autoradiograms.