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. Author manuscript; available in PMC: 2011 May 6.
Published in final edited form as: Mol Biochem Parasitol. 2005 Oct;143(2):135–145. doi: 10.1016/j.molbiopara.2005.05.009

Fig. 4.

Fig. 4

Import and processing of LRNase HIIC precursor protein into isolated mitochondrial vesicles from L. major. Similar results were obtained from mitochondrial vesicles isolated from L. donovani. Lane LC, the 35S-labeled-LRNase HIIC-precursor protein (10% of the input). Lane 1, protein associated with the mitochondrial vesicles after 30 min. Lane 2, corresponding 35S-labeled proteins inside the mitochondrial vesicles after proteinase K digestion. Lane 3, proteins associated with the 0.5 μM valinomycin-treated mitochondrial vesicles after 30 min. Lane 4, same as lane 3 but after proteinase K digestion. The 53-kDa precursor protein (LRNase HIIC) and the 49-kDa mature proteins (LRNase HIICΔMLS) are indicated. LRNase HIIC ΔMLS did not show any significant import to the mitochondrion (data not shown).