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. Author manuscript; available in PMC: 2011 May 6.
Published in final edited form as: Mol Biochem Parasitol. 2005 Oct;143(2):135–145. doi: 10.1016/j.molbiopara.2005.05.009

Fig. 5.

Fig. 5

Mitochondrial ribonuclease H (LRNase HIIC) is essential for the survival of Leishmania. (A) Northern analysis of RNA isolated from L. donovani promastigotes treated for 72 h with the sense or the antisense oligonucleotide (25 μM). Top panel is the autoradiogram and the lower panel corresponds to the ethidium bromide stained gel showing rRNA bands. (B) Activity gel assay with the proteins (100 μg) from the mitochondrial fraction of L. donovani promastigotes treated for 72 h with sense or antisense oligonucleotide (25 μM). Top panel is the zymogram and lower panel is the corresponding Coommasie blue-stained gel. (C) Inhibition of the growth of L. donovani promastigotes by antisense gapmer oligonucleotides against LRNase HIIC. Results are mean ± S.E. (n = 6). Similar results were obtained with axenic amastigotes and L. major promastigotes (not shown). (D) Inhibition of the growth of L. major amastigotes inside cultured macrophages (J774A1) by antisense gapmer oligonucleotides against LRNase HIIC. Results are mean ± S.E. (n = 6). SSN, the sense oligo; INV, oligo with the inverted sequence; SCR, the oligo with the sequence scrambled, and ASN, the antisense oligo. The amastigotes were counted 5-days post-treatment. The counts of amastigotes in the control macrophages (no oligo treatment) were 587 ± 12 cells/100 macrophages.