Table 2.
AA | L. majora | T. brucei | T. cruzi |
---|---|---|---|
RNase HI | 42 kDa (381 aa) LmjF06.0290 (AY835988) | 33 kDa (301 aa) Tb07.26A24.40 | 36 kDa (323 aa) Tc00.1047053506351.40 |
RNase HIIA | 33 kDa (296 aa) LmjF13.0050 (AY835989) | Absent | Absent |
RNase HIIB | 33 kDa (301 aa) LmjF36.0640 (AY835990) | 36 kDa (326 aa) Tb10.70.2140 | 36 kDa (324 aa) Tc00.1047053508661.50 |
RNase HIIC | 53.3 kDa (474 aa) LmjF36.0330 (AF441859) | 54 kDa (477 aa) Tb10.70.2470 | 55 kDa (479 aa) Tc00.1047053503891.70 |
The lengths of the proteins (number of amino acid residues) are given in the parenthesis following the calculated molecular weight. Temporary systematic IDs for the L. major sequences are retrieved from its GeneDBs (release 5.1 for Friedlin, the reference strain MHOM/IL/80/Friedlin, zymodeme MON-103), T. brucei sequences are retrieved from its GeneDBs (Release 4 of T. brucei strain TREU927/4 GUTat10.1 genome; http://www.genedb.org/genedb/tryp/index.jsp) and for the T. cruzi CL Brener (TIGR-Seattle Biomedical Research Institute-Karolinska Institute T. cruzi Sequencing Consortium (TSK-TSC); http://www.genedb.org/genedb/tcruzi/index.jsp) are written below the molecular sizes.
DNA sequences showing the 5′-UTR, transplicing signal, coding sequence and the 3′-UTR for all of the ribonucleases H of L. major are deposited to GenBank. Corresponding accession numbers are shown for each of RNase H in the parenthesis.