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. 2010 Nov 3;25(1):104–116. doi: 10.1210/me.2010-0225

Figure 2.

Figure 2

LH/hCG induces the expression of neuregulin 1 (NRG1) in the mouse ovary during ovulation. A, The temporal changes of Nrg1 mRNA expression in granulosa cells collected from ovaries of mice injected with eCG+hCG. For reference, the 0 h value was set as 1, and the data are presented as fold increase. Values are mean ± SEM of three replicates. *, Significant differences were observed as compared with those in granulosa cells recovered from eCG-primed mice (hCG 0 h), *, P < 0.05. B, The expression of Nrg1 mRNA in cumulus cells of COCs collected from ovaries of mice injected with eCG+hCG. For reference, the 0 h value was set as 1, and the data are presented as fold increase. Values are mean ± SEM of three replicates. C, The expression of Adam19 mRNA in granulosa cells. For reference, the 0 h value was set as 1, and the data are presented as fold increase. Values are mean ± SEM of three replicates. *, Significant differences were observed as compared with those in granulosa cells recovered from eCG-primed mice (hCG 0 h) (P < 0.05). D, Western blot analysis of NRG1 protein level in granulosa cells (GC) or whole ovary recovered from mice injected with eCG+hCG. im, immature mice without any injections. Results in each panel are representative of three separate experiments.