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. 2010 Nov 3;25(1):104–116. doi: 10.1210/me.2010-0225

Figure 6.

Figure 6

The functional roles of NRG1 in AREG/LH-induced granulosa cell luteinization. A, Progesterone production and the expression of genes involved in luteinization (Cyp11a1, Sfrp4, Star) in granulosa cells cultured with 100 ng/ml of AREG, 10 ng/ml of NRG1, or AREG+NRG1 for 4 h (RNA sample) or 8 h (progesterone assay). For reference, the control (C) value was set as 1, and the data are presented as fold increase. Values are mean ± SEM of three replicates. *, Significant differences were observed as compared with that in control (without any agonist) (P < 0.05). B, The relationship between LH-induced luteinization and the EGF-like factors, granulosa cells were cultured with LH and the broad metalloprotease inhibitor galardin (10 μm) to suppress the release of the EGF domain from EGF like factors. Additional effects of AREG (100 ng/ml), NRG1 (10 ng/ml), or both on progesterone production and the expression of genes involved in luteinization (Cyp11a1, Sfrp4, Star) in granulosa cells were analyzed. For reference, the control (C) value was set as 1, and the data are presented as fold increase. Values are mean ± SEM of three replicates. *, Significant differences were observed between the treatment groups (P < 0.05).