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. 2010 Dec 8;25(1):170–183. doi: 10.1210/me.2010-0330

Figure 4.

Figure 4

Abnormal myogenesis in Rxfp2−/− gubernaculum. A, Desmin expression in the wild-type and Rxfp2−/− gubernaculum (arrowheads) revealed by immunohistochemistry. Two layers of muscle cells in the rim of the gubernacular bulb in a wild-type gubernaculum are visible at E15.5. The mutant gubernaculum contains a sharply reduced number of disorganized desmin-positive cells. Scale bar, 100 μm. B, Reduced expression of Notch1, β-catenin, and Wnt1 in the Rxfp2−/− gubernaculum revealed by immunohictochemistry. Note the intense staining for Notch1 and β-catenin in the rim of the gubernacular bulb in wild-type males (brackets). Scale bar, 20 μm. C, Coexpression (yellow) of desmin (green) and β-catenin (red) or Notch1(red) in gubernacular myoblasts revealed by confocal microscopy. Arrows indicate gubernaculum borders. Nuclei are stained with DAPI (blue). Scale bars, 50 μm (top) and 20 μm (bottom). G, gubernaculum; Ep, epididymis; PV, processus vaginalis.

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