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. 2010 Nov 17;25(1):72–82. doi: 10.1210/me.2010-0294

Figure 2.

Figure 2

E2 regulates SGK3 expression via ER at the transcription level. MCF-7 cells were hormone stripped for 48 h before the treatment. After treatment, SGK3 expression was assessed by quantitative RT-PCR (A–D) or Western blotting (E and F). Quantitative RT-PCR was carried out in triplicate and the results were normalized to β-actin. A, The cells were treated with different concentrations of E2 as indicated for 48 h. B, The cells were treated with 10 nm E2 for the indicated time. C, The cells were pretreated with 1 μg/ml actinomycin (ActD) or 100 μg/ml cycloheximide (CHX) or the solvent for 1 h and then treated with 50 nm E2 for 4 h. D, The cells were pretreated with 100 nm ICI182,780 for 1 h and then treated with 10 nm E2 for 9 h. E, The cells were treated with the different concentrations of E2 as indicated for 24 h. F, The cells were pretreated with 100 nm ICI182,780 for 1 h and then treated with 10 nm E2 for 9 h. NS, Nonspecific band.