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. 2010 Nov 24;25(1):92–103. doi: 10.1210/me.2010-0353

Figure 1.

Figure 1

HER2 enhances PRL mRNA expression and secretion. A, GH3 cells stably expressing HER2CA or pcDNA3 (empty vector) were treated with 5 nm EGF or 6 nm HRG for 10 min, and Western blotting was performed. B, GH3 cells stably expressing HER2CA or pcDNA3 were treated with 5 nm EGF or 6 nm HRG for the indicated times, and real-time PCR analysis was performed. C, GH and PRL secretion in the culture medium was determined by RIA. Hormone secretion levels were normalized for cell numbers. Values are mean ± sem. *, P < 0.05; **, P < 0.01. Representative results are from triplicate samples in at least two or more independent experiments.