Fig. 2.

Lipid-induced perturbation Trp fluorescence emission spectrum of VCC (A) and VCC50 (B). The two cytolysin forms were incubated with phosphatidylcholine-cholesterol liposome at a protein concentration of 50 µg/ml and lipid:protein ratio of 1:10. Samples were excited at 295 nm with slit widths set at 2.5 nm.