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. 2011 Feb;133(2):131–137.

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© The Indian Journal of Medical Research

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — Size distribution of VCC and VCC50 micelles with rabbit erythrocyte membrane components and Triton X-100. Following incubation of the toxin with stroma at 25°C, the free toxins were removed by microfuging and the mixture was dispersed in 1 per cent Triton X-100 at 4°C. The suspension was fractionated on Sepharose CL-4B (30 × 0.7 cm) at 4° C. Fractions of 1 ml were collected and quantified for VCC (○) and VCC50 (●) by ELISA (A). Immunoblots of the fractions developed with rabbit anti-VCC antibody and goat anti-rabbit IgG conjugated to alkaline phosphatase are also shown (B).