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Characterization of recombinant proCat. Recombinant proFhCatB was purified from yeast culture supernatant by immobilized metal affinity chromatography using Ni-NTA. The purified protein (arrow) was analyzed by SDS-PAGE (12%) under reducing conditions, followed by silver staining (A) and a gelatin substrate gel (12% gel under nonreducing conditions) (B). Molecular sizes are shown to the right of the gel in panel A.
