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. 2003 Dec;71(12):6884–6891. doi: 10.1128/IAI.71.12.6884-6891.2003

FIG. 2.

FIG. 2.

Western immunoblot assay with a commercial preparation of intravenous immunoglobulin (Gammagard) assayed against cell sonicates of nontypeable H. influenzae isolates strain 12 (lanes 1 to 4) and strain 15 (lanes 5 to 8). Antibody preparations assayed in each are as follows: lane 1, IVIG; lane 2, IVIG adsorbed on strain 12 HMW protein affinity column; lane 3, affinity-purified anti-strain 12 HMW protein antibodies; lane 4, monoclonal antibody 4B2, an antibody that recognizes both the HMW1 and HMW2 proteins; lane 5, IVIG; lane 6, IVIG adsorbed on strain 15 HMW protein affinity column; lane 7, affinity-purified anti-strain 15 HMW protein antibodies; lane 8, monoclonal antibody 1D5.