. 2003 Dec;71(12):6892–6898. doi: 10.1128/IAI.71.12.6892-6898.2003

TABLE 2.

Genes knocked out and methods used

System	ORF name	Homologue	%		Proposed function of homologue	Effect of mutant on TEPC-15 reactivity with pilin	Forward primer	Reverse primer	Insertion site	Construct name
System	ORF name	Homologue	Identity^a	Similarity^a	Proposed function of homologue	Effect of mutant on TEPC-15 reactivity with pilin	Forward primer	Reverse primer	Insertion site	Construct name
Biosynthesis or uptake	NMB1270	pmtA	32	47	Methyltransferase of PEA of R. sphaeroides	−	PmtAFor	PmtARev	ClaI	pGEM-PmtAKan
	NMB1277	betT	35	62	Choline uptake of E. coli	−	BetTFor	BetTRev	BsmI	pGEM-BetTKan
	NMB0939	pmtA	28	42	Methyltransferase of PEA of S. meliloti	−	NMB0939For	NMB0939Rev	BsmI	pGEM-NMB0939Kan
Transferase	NMB0415	lpt-3	27	44	PEA transferase of N. meningitidis	+	NMB0415For	NMB0415Rev	SmaI	pGEM-NMB0415Kan
	NMB1638	lpt-3	26	44	PEA transferase of N. meningitidis	−	NMB1638For	NMB1638Rev	BssHII	pGEM-NMB1638Kan

Percent identity and similarity relative to the following proteins (GenBank accession numbers in parentheses) pmtA, (R. sphaeroides, L07247); betT (X52905); pmtA (S. meliloti, AF201699); and lpt-3 (AE002550).