TABLE 1.
Primers used for PCR amplification of prophage-specific genesa
| Gene | Prophage | Primer
|
Product size (bp) | |
|---|---|---|---|---|
| Forward | Reverse | |||
| ssa | Φ315.2 | TGATCAAATATTGCTCCAAGGTG | TCCACAGGTCAGCTTTTACAG | 502 |
| mf4 | Φ315.3 | TGGCATTGCTTCATAGTAAAGG | ATCTACCTGAAGCTTTGTCGTG | 494 |
| speK | Φ315.4 | GTGTGTCTAATGCCACCGTCT | GGAACATATATGCTCCTAGAT | 564 |
| sla | Φ315.4 | CTCTAATAGCATCGGCTACGC | AATGGAAAATGGCACTGAAAG | 440 |
| speA3 | Φ315.5 | TGGAAAACAATAAAAAAG | TTACTTGGTTGTTAGGTAG | 755 |
| sdn | Φ315.6 | AACGTTCAACAGGCGCTTAC | ACCCCATCGGAAGATAAAGC | 489 |
| speBb | ACCGTGTTATTGTCTATTACC | TGCCTACAACAGCACTTTGG | 1,337 | |
a
Primers are listed in the 5′ → 3′ direction.
b
SpeB is encoded by a chromosomal gene and was used as a negative control.