TABLE 3.
TaqMan primers and probesa
| Geneb | Forward primer | Reverse primer | Probe |
|---|---|---|---|
| speK | GATAAAGACGAAATTTTGGATAATAGAAGA | TTCCAAACTGTAGTATTTTCATCCGTAT | AAATGCCAAACAAGGAACGCAATTGATTTT |
| sla | CGTTGCCAGTAGTAGATGTTTTGG | CAATACCGGCACCCCACTTA | CAAGGTTGCCAAAACCACGATAGTTGCT |
| speA | TTTTTGTTTTAGTGACATTTCTTGGACT | GGAGTTGGCTTGGATCGGG | ACAATCTCGCAAGAGGTATTTGCTCAACAAGA |
| mf4 | GGAGATGCCCTTAGAGTAAATGCT | TCATCCCTCCATTGCCAGA | GACCTCCAACATTTTGTGTTCTTGTTCCTC |
| sdn | GAAACTCTCTCGCCAGAATAGTTGT | TGTACTGTGATGATAAGTTGGAATCG | CAGCATTCGCATCATTTGCTGTGATG |
| ssa | GTGCACAATTATTATCGATTAGTGTTTTT | CACCAGTAAATTGGCTAGATTTGTTTA | CAAGTAGTCAGCCTGACCCTACTCCAGAACAA |
a
Primers are listed in the 5′ → 3′ direction.
b
Gene abbreviations: speK, streptococcal pyrogenic exotoxin K; sla, phospholipase A2; speA, streptococcal pyrogenic exotoxin A; mf4, mitogenic factor 4; sdn, streptodomase; ssa, streptococcal superantigen.