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. 2003 Dec;71(12):6793–6798. doi: 10.1128/IAI.71.12.6793-6798.2003

FIG. 2.

FIG. 2.

Immunoblot analysis of two representative saliva samples for detection of sIgA antibodies directed against wild-type M. catarrhalis O35E and seven isogenic knockout mutants. OMP were separated by SDS-7.5% PAGE, transferred to PVDF membranes, and incubated with saliva samples at a 1:20 dilution. Lane 1, O35E; lane 2, O35E.1 (uspA1 mutant); lane 3, O35E.12 (uspA1 uspA2 double mutant); lane 4, O35E.2 (uspA2 mutant); lane 5, O35E.hag (hag mutant); lane 6, O35E.12-hag (uspA1 uspA2 hag triple mutant); lane 7, O35E.tbpB (tbpB mutant); lane 8, O35E.copB (copB mutant). Numbers to the left of each panel indicate molecular mass standards (in kilodaltons).