FIG. 12.
GroEL is secreted by B. bacilliformis. B. bacilliformis was harvested and radiolabeled for 30 min at 25 or 37°C in HIAB containing 300 μCi of 35S-Express per ml (NEN-Dupont). Culture supernatants were subsequently isolated, separated by SDS-PAGE, and blotted to nitrocellulose, and the immunoblot was developed with anti-GroEL antibody. The blot was then autoradiographed. The resulting autoradiograph is shown and reveals a single radiolabeled 61-kDa protein identified as GroEL (arrow) in the 25°C sample (lane 1) and GroEL plus an ∼10-kDa radiolabeled protein in the 37°C sample (lane 2, parentheses). Molecular mass values are given to the left in kilodaltons.