FIG. 13.

HUVEC numbers correlate with GroEL levels in the Bartonella lysate treatment. HUVEC numbers in response to treatment with B. bacilliformis lysates from strains LSS100 (containing pGRO1, a multicopy plasmid with the groES-groEL operon) and LSS001 (containing the pBBR1-MCS2 vector) cultivated at 30°C or shifted to 37°C for 6 h prior to lysate preparation. Acid phosphatase assays were done after 96 h of HUVEC growth in M199-FCS (20% vol/vol) supplemented with lysates (1.0 μg/ml) from cultures subjected to the two temperature regimens. GroEL levels in the lysates, relative to those in the 30°C vector control, were determined by immunoblot densitometry and are indicated below the corresponding bars of the graph. Data represent the average of three experiments ± standard error.