Immunoblot
showing B. bacilliformis proteins translocated to the growth
medium over a 16-h incubation. Culture supernatants were prepared (see
Materials and Methods), separated by SDS-PAGE (12.5% acylamide),
and blotted to nitrocellulose, and the immunoblot was developed with
anti-B. bacilliformis whole-cell antibody
(32). Lanes: 1, medium
alone; 2, supernatant from the spent growth medium. The positions of
the bacterium-specific proteins, GroEL, flagellin, and the BLPs are
indicated. The identity of the proteins was determined by using
immunoblots developed with monospecific antibodies (not shown).
Molecular mass standards are indicated to the left in
kilodaltons.