FIG. 7.

Immunoblot showing B. bacilliformis proteins translocated to the growth medium over a 16-h incubation. Culture supernatants were prepared (see Materials and Methods), separated by SDS-PAGE (12.5% acylamide), and blotted to nitrocellulose, and the immunoblot was developed with anti-B. bacilliformis whole-cell antibody (32). Lanes: 1, medium alone; 2, supernatant from the spent growth medium. The positions of the bacterium-specific proteins, GroEL, flagellin, and the BLPs are indicated. The identity of the proteins was determined by using immunoblots developed with monospecific antibodies (not shown). Molecular mass standards are indicated to the left in kilodaltons.