FIG. 7.

T cells that recognize fMIGWII also recognize a small protease-resistant molecule present in L. monocytogenes culture supernatant. EL-4 cells were treated with 50 μl of 10403s (wild-type L. monocytogenes [wt Lm]) or NR1-1578 (ΔlemA L. monocytogenes [ΔlemA Lm]) culture supernatant preparations either untreated, digested with proteinase K (protK), or filtered through a 10,000-molecular-weight-cutoff filter and then used as targets for recognition by fMIGWII-specific T cells (line S172) in a ⁵¹Cr release assay. The effector-to-target cell ratio used was 30:1. Average values ± standard deviation from one of two separate experiments are shown.