TABLE 1.
Cytokine concentrations in pleural wash fluids and sera from C57BL/6 (B6) and BALB/c (Bc) mice at D30 p.i.a
| Sample | Expta | No. of mice (B6/Bc) | Concn
 (pg/ml)b | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IL-4 | IL-5 | IL-10 | IFNγ | |||||||
| B6 | Bc | B6 | Bc | B6 | Bc | B6 | Bc | |||
| Pleural wash fluidc | 1 | 6/6 | 2.5 | 0.8 | 24.8 | 1.2 | 296.3 | 7.9 | 90.2 | 22.9 | 
| 2 | 6/6 | 1.9 | 0 | 216.9 | 2 | 18,142.6 | 0 | 309.6 | 36.2 | |
| Serumd | 2 | 6/6 | 0.1 | 0 | 159 | 28 | 18,290 | 0 | 4,084 | 0 | 
| 3 | 8/8 | 8 | 0.2 | 102 | 36 | 15,165 | 525 | 2,660 | 265 | |
| 4 | 6/3 | 2.95 | 0 | 347 | 4.7 | 5,412 | 130 | 5,253 | 844 | |
Four experiments were performed, two for the pleural wash fluid and three for the serum; experiment 2 included both.
Concentrations, measured by two-site ELISA, in infected mice, values for naive mice were subtracted.
The combined results from the two experiments were statistically different (P < 0.01 for IL-4, IL-5, and IL-10 and P = 0.016 for IFN-γ) as calculated by the Mann-Whitney U test.
The combined results from the three experiments were statistically different (P < 0.0001 for IL-4, IL-5, and IFN-γ and P = 0.0002 for IL-10) as calculated by the Mann-Whitney U test.